Thioltrackertm violet dye
WebThe ThiolTracker Violet stain (a registered trademark of Invitrogen) is a bright fluorescent probe that is highly reactive to thiols and can be used as a convenient and effective … WebThe dyes are nonfluorescent in a reduced state and fluoresce bright green, orange, or deep red upon oxidation. Assays are simple and reliable with a sensitive and robust readout, and the reagents can be applied to cells in complete growth medium. ... ThiolTracker™ Violet: Ex/Em (nm) 495/515: 495/515: 405/526: Live cell: No: Yes: Yes: Lysate ...
Thioltrackertm violet dye
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WebJun 7, 2024 · Besides, tumors were stained with the ThiolTracker Violet dye (30 uM) for 40 min and DAPI (1 μg/mL) for 10 min to investigate the cellular level of reduced GSH. Additionally, Anti-Ki67 Rabbit pAb (Servicebio) was used to monitor the cell proliferation of tumor cells according to the manufacturer’s protocol. WebViolet excitation/emission spectra (415/516nm maxima) are optimal for multiplexing with green and red fluorescent dyes and proteins. Easy to use: remove medium, add dye, incubate 30 minutes, and image cells. Fluorescent signal retention of >72 hours (typically three to six generations) Low cytotoxicity: does not affect viability or proliferation.
http://login.qa.thermofisher.com/content/dam/LifeTech/migration/en/filelibrary/cell-tissue-analysis/pdfs-posters-for-cta.par.32354.file.dat/o-076497-thiol-2008-chi-hca.pdf WebThiolTracker™ Violet 是一种更明亮和更稳定的细胞内硫醇探针，适用于可重现的 GSH 检测。. 由于还原型谷胱甘肽代表细胞中的大多数细胞内游离硫醇，因此可利用 …
WebThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of Molecular Probes™ ThiolTracker™ Violet (glutathione detection reagent) Fisher Scientific WebDescription: ThiolTracker Violet dye conjugated to glutathione (GSH) Overview. Exitation ...
WebViolet excitation/emission spectra (415/516nm maxima) are optimal for multiplexing with green and red fluorescent dyes and proteins. Easy to use: remove medium, add dye, …
Web55. A method of treating, inhibiting, reducing, ameliorating, and/or preventing a cancer and or metastasis in a subject comprising administering to a subject an adoptive immunothe clearwater cottages meredith nhWebIf not protected, the oxidation into ROS-scavenger GSH in T-cells using ThiolTrackerTM violet disulphide bridge formation or higher oxidation states, namely dye and flow cytometry. These experiments showed that SFN sulfinylation or sulfonylation will take place. clearwater counseling omaha neWebSep 6, 2024 · Primary keratinocytes were exposed to VOCs (toluene, hexane, acetaldehyde, formaldehyde, acetone, 20 ppmV, each) for 4 hours. 24 hours after exposure they were incubated with ThiolTrackerTM Violet ... clearwater counselling okotoksWebThiolTracker™ Violet Follow these guidelines for preparing and storing ThiolTracker™ Violet dye: Prepare the ThiolTracker™ Violet dye stock solution in DMSO as described in … bluetooth connect macbook to ps4http://login.qa.thermofisher.com/content/dam/LifeTech/migration/en/filelibrary/cell-tissue-analysis/pdfs-posters-for-cta.par.32354.file.dat/o-076497-thiol-2008-chi-hca.pdf clearwater counseling and wellness michiganWebA new thiol reactive probe, ThiolTracker Violet dye: • Cell permeable • React with thiols through irreversible covalent bond formation (bromomethyl group) • Excitable with 405 nm laser or Xenon mercury light sources • Viewable with filter sets for Hoechst 33342 • Clone 9 liver cells (rat, ATCC CRL-1439) • Treatments for 2 hours: clearwater counseling associatesWebThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free … bluetooth connect multiple headphones