Phh21 vector
WebJan 1, 2012 · Vector pHH21, which contains polymerase I promoter (P) and terminator (T) sequences, is incubated with BsmBI, yielding the vector fragment. (2) Insert preparation. (2) Insert preparation. Influenza viral RNAs are RT-PCR amplified with oligonucleotides possessing Bsm BI ( left panel ) or Bsa I ( right panel ) restriction sites. WebJun 1, 2005 · The pHH21 vector was kindly provided by Yoshi Kawaoka and Gerd Hobom. We thank Henry Huang for critical reading of the manuscript, Aaron Briley for technical …
Phh21 vector
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WebJun 23, 2024 · The pHW2000 vector is a bidirectional plasmid that has RNA PolI and PolII promoters for the generation of influenza vRNA and mRNA. Thus, cloning a cDNA copy of …
WebFeb 1, 2011 · It has been reported that the PB2 gene was unstable in commonly available cloning plasmids as well as in standard influenza virus reverse genetics plasmids (e.g., pHH21), which contain high copy... WebEight plasmids containing the RNA polymerase I promoter, a cDNA for each of the eight viral RNA segments, and the RNA polymerase I terminator (pHH21-PB2, pHH21-PB1, pHH21-PA, pHH21-HA,...
WebSep 16, 2015 · This demonstrates that the pHH21 vector, containing the human RNA polymerase I promoter and the mouse RNA polymerase I terminator, is an effective system to generate intact viral RNA segments in living mammalian cells. 2.2. Isolating Endogenously-Assembled DENV 5′–3′U-S1 RNPs WebAug 3, 1999 · Schematic diagram of the generation of RNA polymerase I constructs. cDNAs derived from influenza virus were amplified by PCR, digested with Bsm BI, and cloned into …
WebJun 29, 2024 · Influenza virus infects millions of people worldwide and leads to thousands of deaths and millions in economic loss each year. During the 2014/2015 season circulating human H3N2 viruses acquired a novel mutation in the neuraminidase (NA) protein. This mutation has since fixed in human H3N2 viruses.
WebFeb 11, 2024 · By using a PCR approach, a K612R mutation was introduced into different viral PB1 genes, which were then cloned into the pHH21 vector . The plasmid pHH21-SC09NS F-Luc, for the expression of a viral RNA-like firefly luciferase gene under the control of the human RNA polymerase I promoter, has been reported previously [30,31]. All … northern sweden skiingWebMay 4, 2008 · Influenza virus mRNAs are synthesized by the trimeric viral polymerase using short capped primers obtained by a 'cap-snatching' mechanism. The polymerase PB2 … how to run npm as administratorWebApr 25, 2013 · Similarly, Zhou et al. introduce a technique where apparently all eight gene segments are amplified in a single “multiplex” RT-PCR reaction and then inserted into a modified pHH21 vector by In-Fusion cloning (Clonetech). how to run no os at boot pluto sdrWebNational Center for Biotechnology Information northern sweden ski resortsWebJan 23, 2015 · The genetic instability of the cDNA encoding HA segments of the mentioned strains in standard RG plasmids pHW2000 [ 37] and pHH21 was detected when regularly used E. coli strains were transformed (e.g. DH5α and Top10). northern swimming pool isle of manWebMay 25, 2024 · The vector was amplified using the pHH21-vNA-Luc as a template. “PHH21_F” & “PHH21_R” primers (listed in Table 1) were used to amplify and linearize the … northern swimming pool ramsey iomWebfrom pHW12 (18). This cloning vector contains 225 bp of the human pol I promoter and 33 bp of the murine terminator sequence separated by two BsmBI sites. The pol I promoter and terminator elements are flanked by a truncated immediate– early promoter of the human cytomegalovirus (starting approx- how to run npm install windows