WebpEASY® -Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa. • 5 minutes fast ligation of Pfu-amplified PCR products. WebpEASY -Blunt Cloning Kit 使用前请务必仔细阅读说明书目录号：CB101 保存:Trans1-T1 Phage Resistant Chemically Competent Cell-70℃保存六个月，其它-20℃保存六个月。 *片段为胶回收产物，反应时间取最大值。 5、最佳反应温度：25℃ *若片段是高GC结构，可以37℃反应。 （推荐用PCR 仪控温） ® 试剂盒组成 pEASY- Blunt Cloning Vector (10 …

A general RPA-CRISPR/Cas12a sensing platform for

WebJan 31, 2014 · The EcoR I/Not I digested fragments of pEASY-T1-ADH1B were ligated with pPICZB, resulting in pPICZB-ADH1B. The recombined plasmid was transfected by electroporation of Pichia pastoris. Enzyme purification and assays. Cells were harvested by centrifugation and washed with 50 mM Tris–HCl, pH8.0 (buffer A). The cell paste was … peter chabot risp

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WebThis fragment contains 285 bp upstream of the Hsp-3start codon and was ligated into the T1 vector using the pEASY-T1 Cloning kit (Transgen) (Figure 1, step 2) to generate sufficient material for subsequent manipu- lations. WebContext 1 ... determine the detection limit, PCR and LAMP were performed with tenfold serial dilutions of the plasmid pEASY ® -T1-B637 as template. PCR products were detected by agarose gel elec-... WebpEASY ® - T1 Simple Cloning Vector eliminates the multi-cloning sites of pEASY ®-T1 Cloning Vector. It is designed for cloning and sequencing Taq-amplified PCR products. •5 … peter cetera youtube music