Dsrna elisa j2
WebAnti-dsRNA monoclonal antibody K2 can be used for the detection of dsRNA. It is recommended for (Sandwich-) ELISA and provides an isotoype alternative to Anti-dsRNA monoclonal antibodies J2 and K1. Applications: (Sandwich-)ELISA (1:2 dilution in PBS) , IHC, Dot Blot Specificity: Web11 mar 2024 · Double-stranded RNA (dsRNA) is a virus-encoded signature capable of triggering intracellular Rig-like receptors (RLR) to activate antiviral signaling, but whether intercellular dsRNA structural...
Dsrna elisa j2
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Web本发明涉及一种产生活减毒疫苗的方法。在一些实施方案中，所述方法包括：a)修饰原始病毒以产生至少一种遗传上不同的不适应病毒；b)用所述至少一种不适应病毒感染宿主细胞；c)选择响应于使用所述至少一种不适应病毒进行的所述感染而显示出预选表型的宿主细胞并且从所述宿主细胞中分离所述 ... WebThe anti-dsRNA antibody J2 is suitable for a variety of methods such as electron microscopy, immunofluorescence microscopy, immunohistochemistry blot based methods such as dot blot and western blot as well as ELISA. The property of the antibody to bind well to kerosene fixed cells (IHC-P / FFPE) in immunohistochemistry should also be …
WebThis technology is slow, labour intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment. A well … J2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine fever virus, Brome mosaic virus and many more in cultured cells and also in fixed paraffin-embedded histological samples.
WebJ2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine … WebMouse Anti-dsRNA Monoclonal antibody for IP, ELISA, EM, IF, IHC Bring this labeled antibody directly to your bench! COA SDS Datasheet Summary Documentation References Specifications Antibody Isotype IgG2a, κ Clone sK3 Species Reactivity N/A Immunogen The original J2 antibody was raised by immunising mice with L-dsRNA. Conjugate …
WebApplications Application Notes Mouse monoclonal antibody J2 can be used for ELISA, dsRNA-immunoblotting, immunoaffinity chromatography and in certain systems also for immunohistochemistry. The optimum working dilution of each antibody for any specific application should be established by titration. Target Alternative Names double stranded …
WebThe detection is highly specific: dsRNA in nucleic acid extracts can be detected in the presence of 1.000-10.000 fold excess of other nucleic acids. The assay was based on a sandwich ELISA principle and used a J2 (IgG2a) mouse monoclonal antibody against dsRNA as the capture antibody. Monoclonal antibody K2 (IgM) is used to detect antibodies. new houses for sale swindon wiltshireWebDouble-stranded RNA (dsRNA) plays essential functions in many biological processes, including the activation of innate immune responses and RNA interference. dsRNA also … in the manner of 意味WebJ2 can be used to detect dsRNA intermediates of viruses as diverse as Hepatitis C virus, Dengue virus, rhinovirus, Chikungunya virus, Rabies virus, Polio virus, Classic swine … new houses for sale wirralWebdsRNA (J2) Mouse mAb recognizes dsRNA where the length of the helix is greater than or equal to 40 base pairs (bp). The ability to recognize dsRNA is independent of the sequence and nucleotide composition of the antigen. The J2 clone shows a higher affinity to natural dsRNA than to other dsRNA antigens (6). Species Reactivity: All Species Expected in the manner of speaking chordsWeb25 lug 2024 · c, Immunostaining of dsRNA in HeLa cells with anti-dsRNA (J2) antibody. Mitochondria and nuclei are stained with MitoTracker Deep Red and Hoechst, respectively. Scale bars, 10 μm. new houses for sale worksopWebMouse monoclonal antibodies J2, J5 and K1 can be used for ELISA, dsRNA-immunoblotting, immunoaffinity chromatography and in certain systems also for immunohistochemistry (see references). The optimum working dilution of each antibody for any specific application should be established by titration. new houses for sale whitstable kentWeb1 giu 1991 · DsRNA was readily detected by indirect ELISA (ELISA-I) and dot immunobinding assay (DIA). Addition of the antigen to poly- l -lysine-precoated plates and blocking with uncreamed milk powder allowed detection levels of 100 pg · ml −1 In-Cn by ELISA-I. Concentrations as low as 1 ng · ml −1 were detected by DIA using polyvinyliden … new houses for sale wroughton swindon